Published On May 29, 2012 | Issue 2 | Volume 16 2011

MORFOGENESIS PADA DAUN TANGKAI DAUN DAN RUAS BATANG KENTANG HITAM Solenostemon rotundifolius POIR JK MORTON SECARA IN VITRO

Aryani Leksonowati, Witjaksono
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Cibinong Science Center Jl. Raya Bogor-Jakarta Km 46, Cibinong, Bogor Telp/Fax: (021) 8765066/(021) 8765067
Abstract

Solenostemon rotundifolius (Poir) JK Morton is a minor crop that produce carbohydrate and therefore can be utilized for fooddiversifi cation and help food security program. This crop is not cultivated intensively but it is planted as a side crop during the dryseason. Low yield could be responsible for its unattractiveness for farmer to farm it intensively. Improvement of varieties is needed butit is hindered by the low genetic variability due to lack of sexual reproduction. Manipulation at cell level and biotechnology could be analternative for improving this crop. The availability of a reliable protocol(s) for plant regeneration is needed. This study is intended todevelop reliable regeneration protocols using inocula derived from shoot culture on MS basal medium supplemented with various levelof BA and NAA. The results show that inocula of leaf, petiole, and stem internodes respond classically to the varying concentration ofsitokinin BA and auxin NAA. The optimum production of shoot that refl ect the best shoot organogenic response were achieved by leavesinoculums grew on medium supplemented with 5 mg/l BA and 0.1 mg/l NAA. This protocol serves as a protocol for further somatic cellgenetic experiments to increase genetic variability of this crop.

Key words: in vitro culture, morphogenesis, organogenesis, Solenostemon rotundifolius (Poir) JK Morton

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