Published On Oct 27, 2012 | Issue 1 | Volume 17 2011

EFEK PROTEKSI EKSTRAK AIR PANAS BUAH MAHKOTA DEWA Phaleria macrocarpaSCHEFF BOERL TERHADAP STRES OKSIDASI AKIBAT FERRI SITRAT PADA KHAMIR Candida tropicalis

Heddy Julistiono
Bidang Mikrobiologi, Pusat Penelitian Biologi LIPI, Cibinong Science Center, Indonesia
Abstract

Yeast Candida tropiclis had been used as a cell model to investigate effects of drugs in cell level. Phaleria macrocarpa (Scheff.)  Boerl., is traditionally used in Indonesia as medicinal plant. This study was to evaluate the antioxidant property of P. macrocarpa in  cell level by using yeast C. tropicalis induced with ferric citrate. Ferric citrate of 1 mM or 5 mM induced oxidative stress inthe the  yeast, marked with increasing of an oxidative damage marker, malon dialdehyde (MDA). Concentration of 5 mM of Ferric citrate  caused cell mortality but concentration of 1 mM did not affect cell viability. Hot water extract of P. macrocarpa of 1 mg/ml attenuated  MDA level in yeast cell induced with 1 mM ferric citrate. Whereas, 1 mg/ml of P. macrocarpa decreased cell mortality of the yeast  induced with 5 mM ferric citrate with out decreasing level of MDA. However, 4 mg/ml of P. macrocarpa induced oxidative damage  in yeast cell. The data may indicate the potential use of Indonesian traditional plant P. macrocarpa as herbal medicine for protecting  human cell from oxidation damage.

Key words:Antioxidant, Candida tropicalis, Oxidative Stress, Phaleria macrocarpa

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