Published On May 29, 2012 | Issue 2 | Volume 16 2011

PEMEKATAN EKSOPOLISAKARIDA EPS DARI KULTUR BAKTERI USUS DALAM BIOMASSA SAGU Metroxylon SP MELALUI MODUL MEMBRAN ULTRAFILTRASI CROSSFLOW UFCF

Agustine Susilowati, Aspiyanto, Achmad Dinoto
Pusat Penelitian Kimia - LIPI, Kawasan PUSPIPTEK, Serpong, Tangerang Selatan – 15314, Telephone +62-021-7560929 & E-mail: agustine_1408@yahoo.co.id Pusat Penelitian Biologi - LIPI, Cibinong, Bogor, Telephone +62-021-8765067
Abstract

Cross-fl ow ultrafi ltration (UF) membrane is a main separation and/or concentration techniques infl uenced by operation condition(fl ow rate, temperature, pressure, time) and molecular weight (MW). The goal of this experiment was to fi nd out the effect of concentrationtime of Lactobacillus sp. FU 0811 and Enterobacter sp. FU 0813 cultures in biomass of sago (Metroxylon sp.) on UF membraneperformance (fl ux value and rejection), and retentate/concentrate composition as carrier agent of oral insuline activated compounds.Concentration process was performed through cross-fl ow UF membrane module at pump motor frequency of 20 Hz (fl ow rate of7.5 L/minute), room temperature and operation pressure of 5 bar for each 0, 15, 30 and 45 minutes. The result of experiment showedthat retentate/concentrate had better composition and total microbes count than that permeate. Based on EPS as reduction sugar, timeneeded to concentrate Lactobacillus sp. FU 0811 culture as retentate/concentrate was reached at 30 minutes, with reduction sugar,total solids, total acids concentrations, and total microbes count of 4.2 mg/mL, 0.2443% and 0.013%, and 4.61 log CFU/mL. While,permeate fl ux value was 43.66 L/m2.hour, and rejections of reduction sugar, total solids, total microbes and total acids on membranewere 85.7–90.6%, 96.6–99.1%, 100% dan 12.9–40.2%. Whereas, EPS as reduction sugar, time needed to concentrate Enterobacter sp.FU 0813 culture as retentate/concentrate was reached at 30 minutes, with reduction sugar, total solids, total acids concentrations, andtotal microbes count of 0.18 mg/mL, 0.0901% and 0.013%, and 4.806 log CFU/mL. While, permeate fl ux value was 40.83 L/m2.hour,and rejections of reduction sugar, total solids, total microbes and total acids on membrane were 0–68.75%, 81.4–86.8%, 100%, dan17.6 – 30.3%. Identifi cation of retentate/concentrate from Lactobacillus sp. FU 0811 culture showed that its presence of EPS compoundsas Dextran (MW 10,500 Dalton, Da.), Maltohexaose (MW 342.3 Da.) and Lactose (MW 990.86 Da.)

Key words: Exopolysaccharide (EPS), Lactobacillus sp. FU 0811, Enterobacter sp. FU 0813, Ultrafi ltration (UF), retentate/concentrate,permeate

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