Published On Dec 30, 2018 | Issue 1 | Volume 24 2018

The orbicules and allergenic protein of African tulip tree (Spathodea campanulata P.Beauv.): a roadside ornamental plant in Malang, Indonesia

Janis Damaiyani
Purwodadi Botanic Garden – Indonesian Institute of Sciences, Jl Surabaya-Malang Km 65, Purwodadi, Pasuruan, East Java, Indonesia
Yekti Asih Purwestri
Biology Faculty, Gadjah Mada University, Yogyakarta, Indonesia
Issirep Sumardi
Biology Faculty, Gadjah Mada University, Yogyakarta, Indonesia
Abstract

Orbicules or ubisch bodies are capable carrying specific pollen antigens which responsible for presenting allergenic activity in the atmos-phere. Currently, there is no report on the orbicules and allergenic protein in African tulip tree. Thus, this research was aimed to investi-gate the presence of orbicules and allergenic protein in African tulip tree and to determine their potential as allergen source. The ultra-structure of orbicules were detected using Transmission Electron Microscope (TEM) and Scanning Electron Microscopy (SEM). Aller-genic proteins were analyzed based on the molecular weight using SDS-PAGE. The result showed that he orbicules were present in the tapetal cells. Furthermore, the pro-orbicules were released by tapetal cells and fill the loculus before anthesis. The SDS-PAGE analysis showing several proteins with a molecular weight of 10-70 kD were detected in African tulip tree and cauld be potential as source for allergen. Protein bands with a molecular weight 55 kDa, 48 kDa, 39 kDa, and 27 kDa appear with the darkest, clearest and thickest ex-pression of genes compared to other bands. In other species, the protein bands with molecular weight 55 kDa, 48 kDa and 27 kDa were detected as allergen. This results indicated African tulip tree could be potential for pollen allergen from ultrastructure and allergenic pro-tein observation.

Keyword

African tulip tree, protein expression

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