Published On Oct 27, 2012 | Issue 1 | Volume 17 2011

PENGARUH KEMASAN DAN LAMA PENYIMPANAN Acetobactersp RMG2 DALAM BAHAN PEMBAWA TERHADAP POPULASI DAN PRODUKSI BIOSELULOSA DENGAN KONSENTRASI INOKULUM TERBATAS

Ruth Melliawati
(Editor in Chief), Biology Department, University of Brawijaya
Nuryati Nuryati
Pusat Penelitian Bioteknologi, jl. Raya Bogor KM 46, Cibinong 16911, Bogor
Abstract

Bioselulosa or nata de coco is one food product that contains 25% fiber, which is very useful to support health, especially the  digestive system. Along with the development of science and technology, raw material for making nata de coco become increasingly  diverse as well as developing its usefulness for the wider interests. Inoculum became one of the things that are important in nata  production process is also a determinant of product quality. This research was conducted to obtain information about the packaging is  good for storing inoculum Acetobacter sp. RMG-2 in the carrier material and the efficiency of inoculum for the production bioselulosa.  Two kinds of containers (straws and plastic bags) with two kinds of carrier material (cellulose pulp and CMC) are used. Storageof  inoculum carried out for 20 weeks at 4 ° C. The measured parameter is the number of population, wet weight, dry weight and thickness  as well bioselulosa pesentase minimal inoculum for the manufacture bioselulosa. Result, the inoculum in the CMC are packed using a  straw is better than the plastic bag packaging, with a population of Acetobacter sp RMG-2 at 4.53 × 108 cells/ml, while in the plastic  bag packaging population reached 3.5 x 107 cells/ml, respectively during storage was 20 weeks (five months). Bioselulosa production  uses between 1 €“15 ml inoculum in 1250 ml of medium/tray of wet weight data obtained vary. With 1 ml of inoculum produced an  average wet weight of 725 grams with a thickness of 0.6 cm. Research on the percentage of inoculum between 0.1 to 1.0%, showingthat the use of 0.1% (in the CMC carrier materials for 8 weeks) can still be used for production with the average bioselulosa/100 ml  of medium weight 31.45 grams wet, 1.6 g dry weight and thickness of 0.8 cm during the 7 days incubation. Investigation was still  continuing to be carried out to get optimal results.

Key words: Acetobacter sp. RMG-2, packaging inoculum, The population, the percentage of inoculum

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