Published On Oct 27, 2012 | Issue 1 | Volume 17 2011

ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI HERBISIDA DIURON DAN BROMACIL DARI AREA PERKEBUNAN DI LAMPUNG

Nur Laili
Kawasan CSC, Jl Raya Jakarta-Bogor Km 46 Cibinong 16911
Hartati Imamuddin
Kawasan CSC, Jl Raya Jakarta-Bogor Km 46 Cibinong 16911
Abstract

Diuron and bromacil are a broad-spectrum herbicide that is widely used in some plantation at Lampung. The soil sample is collected  from some locations such as at banana Plantation (NTF), pineapple plantation (GGPC) and sugarcase plantation (GM). The aims of  the research was to know the isolation and characterization of diuron and bromacil degrading bacteria. The highest diuron resistant  bacteria was found in GM soil sample and The highest bromacil resistant bacteria was found in NTF soil sample. The result showed  that there are three isolates for diuron degradation and five isolates for bromacil degradation bacteria from NTF, GGPC and GM. These  isolates were tested to explore their ability for diuron and bromacil degradation on different concentrations. Isolation of bacteria was  tested by using enrichment culture method and degradation of diuron and bromacil were checked by using spectrophotometric method.  Diuron and bromacil degradation by 5 consortium indicated that all of consorsia have the ability to degrade them.

Key words: bacteria, bromacil, characerization, diuron, isolation

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