Published On Mar 07, 2018 | Issue 2 | Volume 23 2018

Isolation and characterization of LEAFY homologous gene partial sequences from Dendrobium capra J.J. Smith Endemic Orchid of Java Island

Muhammad Dylan Lawrie
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Indonesia
Ari Indrianto
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Indonesia
Aziz Purwantoro
Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia
Endang Semiarti
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Indonesia
Abstract

Dendrobium capra is an endemic orchid species on the island of Java, Indonesia, which blooms only once a year, usually in February. In the flowering process, LEAFY (LFY) gene and its homologs are considered as key regulators in flowering of higher plants, including orchids. To understand floral regulatory mechanisms in orchids, information on the flowering gene structure needs to be explored. Partial sequences of LFY homologue of D. capra has been isolated and consists of 220 bp encoding 72 amino acid sequences. Bioinformatic analysis using Blastp search showed high similarity between the LFY genes of D. capra with other orchid LFY genes and has adjacent evolutionary relationship among orchid’sLFY. Amino acid sequence alignment result showed similar characteristic with other orchid’s LFY with conserved motif leucine-rich repeat and DDPRRRLLLLSPDQHN amino acid motif.

Keyword

endemic orchid, genetic diversity

Preview
Download File here