Published On May 29, 2012 | Issue 2 | Volume 16 2011

KEANEKARAGAMAN BAKTERI PENGHASIL EKSOPOLISAKARIDA ASAL SALURAN CERNA MANUSIA

Achmad Dinoto, Sugiyono Saputra, Agustinus Joko Nugroho, Rita Dwi Rahayu
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI). Jl. Raya Jakarta-Bogor Km. 46 Cibinong 16911 Indonesia. Telp. +62-21-8765066 Fax. +62-21-8765062
Abstract

Exopolysaccharide (EPS) is a polysaccharide that is excreted by cell as metabolites. Bacterial EPS has been widely used in food anddrug industries. In order to fi nd natural products for foods and drugs, the study was conducted to know the diversity of EPS-producingbacteria isolated from human gastrointestinal tracts. As much of 30 bacterial strains were collected from two healthy people and seventeenof collected isolates were investigated for the capability of producing EPS. As the results of identifi cation by partial 16S ribosomalDNA analysis, those seventeen EPS-producing bacterial strains were closely related with already known bacteria (range between 86 to100% similarity) and could be classifi ed into seven genus, i.e.: Lactobacillus spp. (9 strains), Enterobacter sp. (2 strains), Escherichiasp. (2 strains), Klebsiella sp. (1 strain), Cronobacter sp. (1 strain), Staphylococcus sp. (1 strain), and Alteromonas sp. (1 strain). Inaddition, member of Lactobacillus group which have closest relationship with species L. plantarum (7 strains) dan L. fermentum (2strains) were known to dominate the collection of culturable EPS-producing bacteria from human gastrointestinal tracts.

Key words: Exopolysaccharide (EPS), bacteria, human gastrointestinal tract, 16S rDNA analysis

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