Published On Oct 27, 2012 | Issue 1 | Volume 17 2011

PENGARUH UMUR BIAK DAN POSISI DAUN TERHADAP MORFOGENESIS DARI DAUN KENTANG HITAM Solenostemon rotundifolius POIR JK Morton IN VITRO

Tatin Suherlina
Pusat Penelitian Biologi LIPI, Cibinong Science Center, Jl Raya Bogor-Jakarta Km 46, Cibinong, Bogor 16911 Telp/Fax: (021) 8765066/(021) 8765067
Aryani Leksonowati
Pusat Penelitian Biologi LIPI, Cibinong Science Center, Jl Raya Bogor-Jakarta Km 46, Cibinong, Bogor 16911 Telp/Fax: (021) 8765066/(021) 8765067
Witjaksono Witjaksono
Pusat Penelitian Biologi LIPI, Cibinong Science Center, Jl Raya Bogor-Jakarta Km 46, Cibinong, Bogor 16911 Telp/Fax: (021) 8765066/(021) 8765067
Abstract

Plant regeneration via somatic embryogenesis or organogenesis is a key for manipulation of somatic cells in vitro. Organogenesis  from leaf inoculum, petiole and stem segments of in vitro black potato (Solenostemon rotundifolius (Poir) JK Morton) is influenced  by the balance of the concentration of BA and NAA in the culture medium. However, organogenesis could also be affected by factors  inherent to the inoculums. This paper reports the influence of leaf position on the in vitro shoots and culture age on the formation and  production of shoot adventives from leaf inoculum. Leaves excised from shoots of 3 €“8 week-old culture and leaf position 1-5 from apical  to basal was used as inocula. The highest percentage (83.3%) of adventitious bud develops at cultures age of 5 weeks with the average  number of shoots 6.1. The highest percentage of bud development occurs at leaf position number 2-4, with the percentage of buds of  83.3, 76.2 and 76.2, respectively with the average number of shoots of 3.19, 2.10 dan 2.38 respectively. Organogenesis that produces  the highest shoot production of 18.6 per Petri dish was obtained from leaves derived from the 2ndleaf from apical of 5 week-old culture  inoculated on MS medium enriched with 5 mg/l BA and 0.1 mg/l NAA.

Key words: culture age, in vitro culture, leaf position, organogenesis, Solenostemon rotundifolius (Poir) JK Morton

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