Published On Oct 26, 2012 | Issue 1 | Volume 17 2011

AMOBILISASI SEL Bacillus licheniformisKA08 DALAM MENGHASILKAN KERATINASE TERMOSTABIL

Anthoni Agustien
Jurusan Biologi, Fakultas MIPA, Universitas Andalas, Padang
Abstract

Isolate local Bacillus licheniformis KA-08 known extracellular thermostable keratinase producers. Scale up of thermostable  keratinase production can be with cells immobilized. The objective of the research is to thermostable keratinase production of B.  licheniformis KA-08 cells immobilization. Thermostable keratinase activities were determined with modification of Brandelli and  Riffel method. Protein concentration of enzyme determined with Lowry method. Immobilization of cells by Ca-alginate matrix with Adinarayana method, alginate concentration and amount of alginate bead effects with Beshay method. The result extracellular  thermostable keratinase of B. licheniformis KA-08 cells immobilized was maximum produced at 12 times incubation with activity as  9.25 U/mg. Three percent alginate has optimum activity. Three hundred alginate beads has optimum activity. Cells immobilized ofB.  licheniformis KA-08 has scale up of thermostable keratinase activity at 2 times than free cells. Thermostable keratinase produced by  cell immobilized was nine cycles.

Key words: alginate, B. licheniformis KA-08, immobilization, keratinase, thermostable

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