Anthoni Agustien


Isolate local Bacillus licheniformis KA-08 known extracellular thermostable keratinase producers. Scale up of thermostable keratinase production can be with cells immobilized. The objective of the research is to thermostable keratinase production of B. licheniformis KA-08 cells immobilization. Thermostable keratinase activities were determined with modification of Brandelli and Riffel method. Protein concentration of enzyme determined with Lowry method. Immobilization of cells by Ca-alginate matrix with Adinarayana method, alginate concentration and amount of alginate bead effects with Beshay method. The result extracellular thermostable keratinase of B. licheniformis KA-08 cells immobilized was maximum produced at 12 times incubation with activity as 9.25 U/mg. Three percent alginate has optimum activity. Three hundred alginate beads has optimum activity. Cells immobilized of B. licheniformis KA-08 has scale up of thermostable keratinase activity at 2 times than free cells. Thermostable keratinase produced by cell immobilized was nine cycles.

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alginate, B. licheniformis KA-08, immobilization, keratinase, thermostable

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How to Cite
Agustien, A. (2012). AMOBILISASI SEL Bacillus licheniformis KA-08 DALAM MENGHASILKAN KERATINASE TERMOSTABIL. Berkala Penelitian Hayati, 17(1), 33-36. https://doi.org/10.23869/185