Exploration of synthetic dyes degrading bacteria from Siak River, Riau Province

Abstract

The presence of dyes causes a decrease in river quality. Degradation using bacteria could be used as an alternative method to overcome the dyes substances waste. This research aimed to isolate bacteria that can degrade dyes such as congo red (CR) and methylene blue (MB) from Siak River. The result of this study identified a total of 27 isolates of bacteria that can degrade CR with a concentration of 100 ppm in a Minimal Medium with three days of incubation. The ability of bacteria to degrade CR was known by forming clear zones around the colonies. The degradation index ranged from 1.54 to 5.61 after three days of incubation. The isolate CR26 had the highest degradation. Meanwhile, the number of bacteria that degrades the MB in the concentration of 25 ppm are three isolates (MB1, MB2, and MB3) in the degradation index range from 1.32-1.41. One isolate (MB2) was obtained from the concentrations of 50 ppm and 100 ppm with a degradation index of 1.42 and 1.34. Identification results isolate CR 26 as Bacillus sp and isolate MB2 as Enterobacter sp. This bacteria can be developed to overcome water pollution caused by CR and MB dyes.