Protocorm Like Bodies (PLB) Growth of Dendrobium sp. Orchid with Induction of Oxygen Nanobubbles (NBsO2) by In Vitro

Abstract

Nanobubbles (NBs) are a technology applicable in various sectors and have proven to be able to accelerate plant germination and growth and increase agricultural yields. The use of NBs in tissue culture still needs to be done. Tissue culture is a technology that can produce a large number of seeds in a short time especially for orchid plants. NBs in orchid tissue culture are expected to accelerate orchid growth. This research aims to identify the growth of Protocorm Like Bodies (PLB) of Dendrobium sp. orchids induced by NBsO₂, and to determine the effects of NBsO₂ on the growth of Dendrobium sp. orchids. The research method involves various steps such as sterilization, making pre-treatment media, making liquid media, sterilizing tools and materials, sterilizing nanogenerators, making NBsO₂ solutions, screening NBs, pre-treatment, induction of Nanobubbles, and measuring plants. The observation parameters include PLB weight (g), PLB height (cm), number of living PLB (%), PLB color, root emergence, root length, shoot emergence, number of new shoots, embryo emergence, and number of embryos. The results showed that the growth of Dendrobium sp. orchid PLB induced by NBsO₂ experienced a rapid and significant growth rate compared to treatments that were not induced by NBsO₂. The use of NBsO₂ affects the growth of Dendrobium sp. orchids by increasing the time of embryo emergence, number of embryos, plant weight, plant height, color, and percentage of life to increase the accelerated growth rate of Dendrobium sp. orchid PLB.