Extraction, Identification, and In Silico Analysis Bioactive Compound of Streptomyces bungoensis strain 15721 from Euphorbia sp. Rhizosphere as Antibacterial and Antiviral Drugs Candidates

Abstract

Actinomycetes are a group of bacteria that display an important role in their ability to produce secondary metabolites in the form of bioactive compounds with various chemical structures and biological activities. Thousands of bioactive compounds have been isolated and characterized. These compounds have been developed into medicines for the treatment of various diseases in humans and animals problems. Antibiotic resistance is still a major global health challenge. This involves the transfer of bacteria and genes between humans, animal dan the environment. The next stage after the exploration of antimicrobial potential is the extraction and purification of the components of bioactive compounds. Therefore, the extraction and identification of actinomycetes bioactive compounds is very important especially in the development of new candidates for antibiotics. Streptomyces bungoensis strain 15721 was previously isolated from Euphorbia sp. Rhizosphere. The methodology comprises from the preparation of the isolate, production and extraction of bioactive compound, antibacterial test and GC-MS analysis. Crude extract showed an average of 12 mm inhibition zone against Escherichia coli ATCC 8739. There are seven bioactive compounds from Streptomyces bungoensis strain 15721. These compounds were Dibutoxy (Dimethyl) Silane, Naphthalene, Butane, 1,1-Dibutoxy, Heneicosane, 2,6,10,14-Tetramethyl-Hexadecane, Icosane and Nonadecane. Bioactive compounds were then analyzed in silico with molecular docking. In silico studies showed that bioactive compounds have very good antibacterial activity with different binding energies, inhibition constants, and protein-ligand interactions. Three compound 2,6,10,14-Tetramethyl-Hexadecane, Naphtalene, and Butane, 1,1 dibutoxy showed promising antibacterial and antiviral activity. These compounds should be further investigated for future antibacterial and antiviral candidates