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Aline Puspita Kusumadjaja Tutuk Budiati Sajidan Ni Nyoman Tri Puspaningsih

Abstract

Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17. Crude thermophilic phytase isolated from Bacillus sp. AP-17 had the optimum temperature at 75° C with activity of 0.1413 U/ml, and its optimum pH was at pH 6 with activity of 0.0875 U/ml. The enzyme was stable when heated at 75° C for three hours and still had 90% activity when it was exposed at pH 5–8, optimum temperature, for one hour.

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Keywords

Bacillus sp., AP-17, crude thermophilic phytase, optimum temperature, optimum pH

References
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How to Cite
Kusumadjaja, A. P., Budiati, T., Sajidan, & Puspaningsih, N. N. T. (2010). KARAKTERISASI EKSTRAK KASAR FITASE TERMOFILIK DARI BAKTERI KAWAH IJEN BANYUWANGI, ISOLAT AP-17. Berkala Penelitian Hayati, 16(1), 9-14. https://doi.org/10.23869/277
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