ANALISIS 6 DNA REKOMBINAN DENGAN ENZIM EcoR1

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Accepted Ni Nyoman Tri Puspaningsih 3(1) 1997
views: 430 | downloads: 224
Published Jan 30, 2014
DOI 10.23869/490

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Ni Nyoman Tri Puspaningsih
FMIPA Universitas Airlangga
Akhmaloka
FMIPA Institut Teknologi Bandung
Afaf Baktir
FMIPA Universitas Airlangga
Ami Soewandi J.S.
FMIPA Universitas Airlangga
Y. Sriwulan M.
FMIPA Universitas Airlangga

Abstract

Amylase enzyme from Endomycopsis fibuligera capable to hidrolize starch into glucose. Insertion of amylase gene from Endomycopsis fibuligera into yeast (Saccharomyces cereviceae) will be able to increase the function of yeast (S.cereviceae) to digest more cheaper substrate, like starch. Before cloning in yeast, recombinant DNA will be made and analyzed in Escherichia coli strain DH5a. The result showed that the sixth transformant consist recombinant DNA that were sensitive to tetracycline medium. Analysis by EcoR1 digestion showed that the size of insertion fragment into Ycp 50 vector are around 0.3 untill 16 kb.

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Keywords

amylase gene, cloning, EcoR1 digestion

References
Citation Format
How to Cite
Puspaningsih, N. N. T., Akhmaloka, Baktir, A., J.S., A. S., & M., Y. S. (2014). ANALISIS 6 DNA REKOMBINAN DENGAN ENZIM EcoR1. Berkala Penelitian Hayati, 3(1), 1. https://doi.org/10.23869/490
Issue
Vol 3 No 1 (1997): June 1997
Section
Articles